BACKGROUND: An organ transplant replaces the failing organ with a healthy organ. Organs most often transplanted include:
The kidney, resulting from diabetes, lupus, polycystic kidney disease, or other problems
The liver, resulting from cirrhosis
The pancreas, resulting from diabetes
The heart, resulting from cardiomyopathy, coronary artery disease, heart failure, and other heart complications.
The lung, resulting from COPD, cystic fibrosis, and other problems.
The small intestine, resulting from short bowel syndrome caused by necrotizing enterocolitis, Crohn’s disease, and other problems.
Not everyone is a good candidate for an organ transplant. Infection, drug or alcohol abuse, heart disease that is out of control and other serious health problems will disqualify someone from receiving an organ transplant. The United States has been doing organ transplants since the 1950s. The procedure has not stopped improving since then.
HOW TO PREPARE: The first step in an organ transplant procedure is finding a match by conducting blood and tissue tests. This is done because the immune system may detect the new organ as foreign and reject it. The more matches with the donor, the greater the chances of the body accepting the donor organ. Patients who qualify for a transplant are advised to continue their medications, get regular blood tests, and to talk to a psychologist or psychiatrist about the transplant.
NEW TECHNOLOGY: Stem cells have been all over the news in the past and there is speculation over using stem cells to help with a serious disease. Stem cells are the body’s raw materials (cells from which all other cells with specialized functions are generated from). Stem cells can divide to form daughter cells. The daughter cells can then become new stem cells or specialized cells with a more specific function, like brain cells, heart muscle, blood cells, or bone. Stem cells can come from amniotic fluid, adult cells altered to have properties of embryonic stem cells, adult stem cells, and embryonic stem cells.
In 2007, scientists discovered that stem cells can be found in amniotic fluid that’s discarded after birth. Now researchers are able to take the stem cells from amniotic fluid to help fix a dying organ. This could provide an alternative to organ transplantation. Researchers hope that the amniotic cells can be frozen and banked in the same way blood is, and patients in need of blood vessel repair would be able to receive the cells through an injection. Also, they hope that the cells could be injected right into the dying organ to help repair it. They hope the cells could regenerate the tissues in the kidney and become part of the kidney. (Source: www.weill.cornell.edu)
Dr. Roger De Filippo, principal investigator at The Saban Research Institute of Children’s Hospital Los Angeles, talks about building organs in the laboratory.
So you see firsthand how a kidney and the failure of kidneys can affect someone from birth?
Dr. De Filippo: Yes. We deal with a lot of congenital conditions. Kids that are born with conditions that would mean significant compromise to their kidney function. We operate on a lot of children that have conditions of the kidney that require extensive reconstruction. It is a part of what we do every day.
A lot of kids come through your hospital that would really benefit from kidney transplants and have to stay on the list for a long time. Do you see their health fail?
Dr. De Filippo: There are a lot of patients today waiting on a transplant list for kidney transplant. While they are waiting, they require dialysis. Having research that could potentially offer them other alternatives is worth pursuing.
And part of that is regenerative medicine right?
Dr. De Filippo: Certainly; regenerative medicine, bioengineering, tissue engineering, all of that is an important aspect of the work that we are doing in the laboratory.
Can you tell me a little bit about that the kidney that you are building in the lab?
Dr. De Filippo: When we first started here, nearly 10 years ago, one of our focuses was regenerating kidneys in the laboratory. So we were actually able to do that with kidneys on a small scale, using stem cells that we were able to retrieve from amniotic fluid. Amniotic fluid is a very good source of stem cells and other cells that lend themselves to regenerative medicine, tissue engineering, and bioengineering. That was actually one of our first projects in looking at our ability to use concepts in embryology and developmental biology to reconstruct these kidneys. We were actually able to insert these cells into small developing kidneys and what we found was that these cells were able to regenerate tissue and would become part of the kidney.
Was that a surprise?
Dr. De Filippo: Well it had never been done before. We were hypothetically thinking that that would happen, but it certainly was a pleasant surprise to see it occur.
Is it just regenerating cells in your own kidney?
Dr. De Filippo: We have 2 basic lines of research in our laboratory; one very classic tissue engineering line where we are building kidneys with scaffolds using regenerative cells. These are the cells that were destined to become various parts of the kidney that we were able to isolate from amniotic fluid. We are also using a cell therapy, where we are able to inject these cells into damaged kidneys or kidneys that would eventually succumb to their disease and fail, and we are able to stave off organ failure.
Does it stop the disease in its progression? What types of diseases are you talking about?
Dr. De Filippo: We have a model right now for chronic kidney disease, which we are working on. We are replicating conditions that in a human would require dialysis. What we are finding is using this cell therapy; we are actually able to slow down the progression of the disease pretty significantly.
Dr. De Filippo: We have some models right now that have received recurrent therapies and that we have been able to basically preserve their renal function to where they are not having any signs or any significant signs, of end stage disease in other words.
Does that suggest that in the future, using these therapies, someone could be able to get off of dialysis?
Dr. De Filippo: That is certainly our hope to delay the need for dialysis quite significantly. That alone would be a very significant improvement over what we have now. The longer you can delay dialysis, the better it is for the patient. An analogy could be someone taking insulin for diabetes. If we could give our patients a therapy that would be similar to that, so that they would not succumb to their disease so early; that certainly would allow them to live a normal life with their disease.
Do you see these future therapies cutting down on the need for transplant?